Antibody development to identify components of IIS and mTOR signaling pathways in lepidopteran species, a set of non-model insects
Abstract
Nutritional stress impacts many insect species that have differing reproductive strategies and life histories, yet it is unclear how nutrient-sensing signaling pathways mediate tissue-specific responses to changes in dietary input. In Drosophila melanogaster, insulin/insulin-like growth factor (IIS) and mTOR-mediated signaling within adipocytes regulates oogenesis. To facilitate comparative study of nutrient-sensing pathway activity in the fat body, we developed antibodies to assess IIS (anti-FOXO) and mTOR signaling (anti-TOR) across three µP microPublication Biology Submit a microPublication nymphalid species (Lepidoptera). By optimizing whole-mount fat body immunostaining, we find FOXO nuclear enrichment in adult adipocytes, like that observed in Drosophila. Additionally, we show a previously uncharacterized TOR localization pattern in the fat body. µP microPublication Biology Submit a microPublication
Local Knowledge Graph (10 entities)
Knowledge graph centered on Antibody development to identify components of IIS with 11 nodes and 25 connections. Top connected: The effect of adult food limitation on life histor, Carol L. Boggs, early-life trade-offs, insulin/insulin-like growth factor signaling, mTOR signaling.
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References (33)
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