Soil nucleic acid extraction with phenol-chloroform (Asteraceae)

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Description

Modified phenol-chloroform extraction protocol optimized for soil samples using CTAB buffer and mechanical disruption for simultaneous DNA and RNA recovery.

Typical Equipment

• 2 ml Lysing Matrix E tube
• MP Biomedicals FastPrep-24 instrument
• AllPrep DNA/RNA Mini Kit

Output Measurements

• purified DNA
• purified RNA
• nucleic acid concentrations

Papers Using This Protocol (1)