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skeletochronology (Ambystomatidae)

Subcategory: histological age analysis
Standard reference: Castanet and Smirina, 1990
Papers: 1 | Mentions: 1

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Knowledge graph centered on skeletochronology (Ambystomatidae) with 13 nodes and 24 connections. Top connected: facultative paedomorphosis, mark-recapture (Ambystomatidae), population stage structure, skeletochronology, lines of arrested growth.

Description

Standard skeletochronology protocol involving tissue fixation, decalcification, cryostat sectioning, histological staining, and LAG counting to determine age in amphibians.

Typical Equipment

  • 70% ethanol
  • 3% nitric acid
  • cryostat (HM 505 E)
  • Harris' haematoxylin stain
  • phase-contrast microscope

Output Measurements

  • estimated age in years
  • LAG count

Papers Using This Protocol (1)

article

Accuracy assessment of skeletochronology in the Arizona tiger salamander (<i>Ambystoma tigrinum nebulosum</i>)

2007Copeiausing