Bumble bee pathogen microscopy survey (Apidae)

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Knowledge graph centered on Bumble bee pathogen microscopy survey (Apidae) with 9 nodes and 16 connections. Top connected: Bees per person hour sampling, plant-pathogen interactions, horizontal gene transfer, infection prevalence, vertical transmission.

Description

Dissection of bumble bees followed by microscopic examination of gut and fat body tissues for protozoan parasites using Giemsa staining. Quantifies pathogen intensity by counting cells in standardized fields of view.

Typical Equipment

dissection tools
microscope slides
methanol
Giemsa solution
phosphate buffer
water
compound microscope

Output Measurements

pathogen presence/absence
pathogen intensity (cell counts)
infection prevalence

Papers Using This Protocol (1)

student_paper

Rocky Mountain Bombus pathogen survey: Are invasive plants affecting pathogen prevalence and intensity?

2008using