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Bacterial endophyte isolation from crucifer leaves (Brassicaceae)
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Knowledge graph centered on Bacterial endophyte isolation from crucifer leaves (Brassicaceae) with 9 nodes and 17 connections. Top connected: Grasshopper feeding bioassay for plant defense, jasmonic acid-salicylic acid crosstalk, leaf mining behavior, cross-resistance, coronatine.
Description
Surface sterilization of plant leaves followed by tissue plating on selective media to isolate bacterial endophytes and assess their growth characteristics and pathogenicity.
Typical Equipment
- cork borer
- Kings B media agar
- 70% ethanol
- blunt syringe
- solid agar plates
Output Measurements
- bacterial colony counts
- growth rate
- virulence symptoms