← Back to Protocolslaboratory

DAB staining (Drosophilidae)

Subcategory: plant defense response
Standard reference: Clarke 2009
Papers: 1 | Mentions: 1

Local Knowledge Graph (18 entities)

Loading graph...

Knowledge graph centered on DAB staining (Drosophilidae) with 18 nodes and 39 connections. Top connected: neighborhood effects, glucosinolates, jasmonic acid-salicylic acid crosstalk, leaf mining behavior, mitochondrial DNA barcoding (Drosophilidae).

Description

Histochemical staining using DAB for hydrogen peroxide and NBT for superoxide detection in plant tissues following herbivore attack. Reveals spatial patterns of oxidative stress responses around feeding damage sites.

Typical Equipment

  • 3,3'-diaminobenzidine tetrahydrochloride (DAB)
  • nitro blue tetrazolium (NBT)
  • microscope

Output Measurements

  • H₂O₂ localization
  • superoxide localization

Papers Using This Protocol (1)

article

Mining the plant-insect interface with a leafmining <i>Drosophila</i> of <i>Arabidopsis</i>

2011Molecular Ecologyusing