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Microsatellite enrichment and primer development (Polemoniaceae)

Subcategory: molecular
Standard reference: Glenn and Schable (2005)
Papers: 1 | Mentions: 1

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Knowledge graph centered on Microsatellite enrichment and primer development (Polemoniaceae) with 11 nodes and 19 connections. Top connected: population structure, microsatellite DNA analysis, Hardy-Weinberg equilibrium, heterozygote deficiency, Microsatellite genotyping (Polemoniaceae).

Description

Development of microsatellite primers through genomic library construction, enrichment, cloning, sequencing, and primer design for population genetic studies.

Typical Equipment

  • RsaI endonuclease
  • biotinylated oligonucleotides
  • TOPO TA Cloning vector
  • Escherichia coli TOP10 competent cells
  • Primer3 software

Output Measurements

  • microsatellite primer sequences
  • allele sizes
  • polymorphism metrics

Papers Using This Protocol (1)

article

Microsatellite loci in <i>Ipomopsis aggregata</i> (Polemoniaceae) and cross-species applicability for ecological genetics studies

2012American Journal of Botanyintroducing