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16S rRNA qPCR detection of skin mites (Vespertilionidae)

Subcategory: molecular
Standard reference: Ferreira et al. [5]
Papers: 1 | Mentions: 1

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Knowledge graph centered on 16S rRNA qPCR detection of skin mites (Vespertilionidae) with 14 nodes and 29 connections. Top connected: Detection, prevalence and phylogenetic relationshi, gene coalescence, plant-pathogen interactions, metabarcoding, population genomic divergence.

Description

Real-time PCR using universal 16S primers to detect mite DNA in mammalian hair samples, with melting curve analysis for specificity assessment.

Typical Equipment

  • Roche LightCycler 480 Real-Time PCR System
  • FastStart Universal SYBR Green Master

Output Measurements

  • mite DNA presence/absence
  • melting temperature
  • amplification cycles

Papers Using This Protocol (1)

article

Detection, prevalence and phylogenetic relationships of <i>Demodex</i> spp and further skin prostigmata mites (Acari, Arachnida) in wild and domestic mammals

2016PLoS ONEusing