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Acute restraint stress protocol (Ambystomatidae)

Subcategory: stress induction
Standard reference: Romero and Reed, 2005
Papers: 1 | Mentions: 1

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Knowledge graph centered on Acute restraint stress protocol (Ambystomatidae) with 8 nodes and 13 connections. Top connected: interspecific competition, facultative paedomorphosis, stress hormones, non-invasive sampling, Dermal corticosterone sampling (Ambystomatidae).

Description

Standardized protocol for inducing acute stress in amphibians through manual restraint to measure stress hormone response over time. Includes baseline sampling followed by stress induction and temporal monitoring of stress hormone levels.

Typical Equipment

  • dip nets
  • plastic buckets
  • cotton-tipped wooden swab precut to 4 cm

Output Measurements

  • stress hormone response curves
  • baseline corticosterone
  • stress-induced corticosterone

Papers Using This Protocol (1)

student_paper

Morph- and sex-specific differences in corticosterone of Arizona tiger salamanders (<i>Ambystoma mavortium nebulosum</i>)

2020using