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Dermal corticosterone sampling (Ambystomatidae)

Subcategory: stress hormone sampling
Standard reference: Santymire et al., 2018
Papers: 1 | Mentions: 1

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Knowledge graph centered on Dermal corticosterone sampling (Ambystomatidae) with 8 nodes and 13 connections. Top connected: interspecific competition, facultative paedomorphosis, stress hormones, non-invasive sampling, Acute restraint stress protocol (Ambystomatidae).

Description

Non-invasive sampling method using swabs to collect dermal secretions containing corticosterone from amphibian skin for stress hormone analysis. This method was validated for use with axolotls and applied to field sampling of salamanders.

Typical Equipment

  • powder-free latex gloves
  • cotton-tipped wooden swab precut to 4 cm
  • 2 mL sterile plastic vials
  • 70% ethanol
  • Corticosterone ELISA kits (Arbor Assays)

Output Measurements

  • corticosterone concentration

Papers Using This Protocol (1)

student_paper

Morph- and sex-specific differences in corticosterone of Arizona tiger salamanders (<i>Ambystoma mavortium nebulosum</i>)

2020using