Bacterial culture isolation and maintenance from plant phyllosphere (Pseudomonadaceae)

Local Knowledge Graph (11 entities)

Knowledge graph centered on Bacterial culture isolation and maintenance from plant phyllosphere (Pseudomonadaceae) with 11 nodes and 19 connections. Top connected: plant-microbial coupling, genome annotation, genome polishing, antimicrobial resistance, ONT long-read genome assembly with dual assembler approach (Pieridae).

Description

Protocol for isolating and maintaining pure bacterial cultures from plant leaf surfaces through surface sterilization, plating on selective media, and cryopreservation in glycerol stocks.

Typical Equipment

• King's B (KB) agar plates
• KB broth
• shaking incubator
• freezer

Output Measurements

• pure bacterial cultures
• frozen stock cultures

Papers Using This Protocol (1)