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CTAB DNA extraction (Pinaceae)

Subcategory: phylloplane microbiome
Standard reference: Carper et al. [20]
Papers: 1 | Mentions: 1

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Knowledge graph centered on CTAB DNA extraction (Pinaceae) with 12 nodes and 26 connections. Top connected: plant-microbial coupling, metagenome-assembled genome, host specificity, horizontal gene transfer, host-microbiome interactions.

Description

Modified CTAB DNA extraction protocol specifically designed to isolate microbial cells from conifer needle surfaces while excluding endophytic microorganisms from internal needle tissues.

Typical Equipment

  • sterile razor blades
  • sterile bags
  • PBS-S buffer
  • vortex
  • sonicator
  • centrifuge
  • CTAB reagents

Output Measurements

  • phylloplane microbial DNA

Papers Using This Protocol (1)

article

Host species and geographic location shape microbial diversity and functional potential in the conifer needle microbiome

2025Microbiomeusing