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COI DNA barcoding (Baetidae)

Subcategory: molecular
Standard reference: Folmer et al. (1994)
Papers: 2 | Mentions: 2

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Knowledge graph centered on COI DNA barcoding (Baetidae) with 20 nodes and 47 connections. Top connected: phenotypic plasticity, Genetic structure in a montane mayfly <i>Baetis bi, benthic sampling, ecological speciation, metapopulation structure.

Description

DNA extraction using modified Chelex protocols followed by PCR amplification and sequencing of the mitochondrial cytochrome oxidase I gene to assess genetic variation within and among mayfly populations.

Typical Equipment

  • Eppendorf tubes
  • chelex solution
  • proteinase K
  • PCR equipment
  • LCO1490 primer
  • HCO2198 primer
  • Applied Biosystems 377 automated sequencer

Output Measurements

  • DNA sequences
  • haplotype frequencies
  • sequence divergence

Papers Using This Protocol (2)

article

Are populations of mayflies living in adjacent fish and fishless streams genetically differentiated?

2005Freshwater Biologyusing
article

Genetic structure in a montane mayfly <i>Baetis bicaudatus</i> (Ephemeroptera: Baetidae), from the Rocky Mountains, Colorado

2003Freshwater Biologyusing