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Standard plant histology (Polemoniaceae)

Subcategory: histology
Standard reference: O'Brien and McCully, 1981
Papers: 1 | Mentions: 1

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Knowledge graph centered on Standard plant histology (Polemoniaceae) with 13 nodes and 27 connections. Top connected: Self-sterility in <i>Ipomopsis aggregata</i> (Pole, pollination exclusion experiment, ecological speciation, self-pollination, self-incompatibility.

Description

Standard plant histological protocol involving glutaraldehyde fixation, resin embedding, serial sectioning at 1.5 μm, and multiple staining techniques including toluidine blue for general structure and specialized stains for cell wall components.

Typical Equipment

  • 3% glutaraldehyde
  • 0.1 M sodium cacodylate buffer
  • ethanol series
  • Spurr's resin
  • microtome
  • toluidine blue
  • ether aniline blue
  • phloroglucinol
  • ruthenium red

Output Measurements

  • cellular structure
  • cell wall composition
  • tissue organization

Papers Using This Protocol (1)

article

Self-sterility in <i>Ipomopsis aggregata</i> (Polemoniaceae) is due to prezygotic ovule degeneration

2006American Journal of Botanyusing