16S rRNA gene clone library (Bacteria)

Local Knowledge Graph (14 entities)

Knowledge graph centered on 16S rRNA gene clone library (Bacteria) with 14 nodes and 30 connections. Top connected: Microbes on mountainsides: contrasting elevational, elevational gradients, phylogenetic signal, 5TE soil sensor monitoring, trait filtering.

Description

PCR amplification of Acidobacteria 16S rRNA genes from soil DNA followed by cloning into vectors and Sanger sequencing to characterize bacterial community composition and phylogenetic diversity.

Typical Equipment

• MoBio Power Soil DNA Isolation kits
• Acidobacteria-specific PCR primers
• TOPO-TA cloning kit
• MinElute PCR Purification Kit
• phyloML program
• PHYLIP program
• DOTUR program

Output Measurements

• 16S rRNA gene sequences
• bacterial phylotypes
• phylogenetic diversity

Papers Using This Protocol (1)