Stigma lobe removal fertilization experiment (Geraniaceae)

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Knowledge graph centered on Stigma lobe removal fertilization experiment (Geraniaceae) with 8 nodes and 18 connections. Top connected: Hemacytometer pollen counting, pollen limitation, self-pollination, gynodioecy, stigma lobe-ovule relationship.

Description

Experimental removal of 0-5 stigma lobes from gynodioecious Geranium flowers to test the relationship between stigma lobe pollination and ovule fertilization. Includes emasculation of hermaphrodites to prevent self-fertilization.

Typical Equipment

DEER-X protective deer fencing
flagging
jewelry tags
forceps
clippers
small coin envelopes

Output Measurements

seed set per treatment
pollen grain counts per stigma lobe

Papers Using This Protocol (1)

student_paper

Effects of quantity and distribution of pollen on fertilization in the gynodioecious species <i>Geranium richardsonii</i>

2009introducing