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Microsatellite genotyping (Polemoniaceae)
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Knowledge graph centered on Microsatellite genotyping (Polemoniaceae) with 27 nodes and 64 connections. Top connected: pollinator foraging behavior, Bagged flower nectar accumulation, photosynthetic rate, ecological speciation, Leaf physiology reflects environmental differences.
Description
PCR amplification of microsatellite loci followed by capillary electrophoresis for allele sizing and polymorphism assessment.
Typical Equipment
- Eppendorf Mastercycler thermocyclers
- ABI 3730xl sequencer
- ROX 500 size standard
- GeneMarker software
Output Measurements
- allele sizes
- heterozygosity
- number of alleles per locus
Papers Using This Protocol (3)
article
Microsatellite loci in <i>Ipomopsis aggregata</i> (Polemoniaceae) and cross-species applicability for ecological genetics studies
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Genetic and morphological patterns show variation in frequency of hybrids between <i>Ipomopsis</i> (Polemoneaceae) zones of sympatry
article