Transgenic functional complementation assay (Brassicaceae)

Local Knowledge Graph (16 entities)

Knowledge graph centered on Transgenic functional complementation assay (Brassicaceae) with 16 nodes and 35 connections. Top connected: herbivory, fitness consequences, glucosinolates, Grasshopper feeding bioassay for plant defense, pleiotropic effects.

Description

Creation and analysis of transgenic Arabidopsis lines expressing candidate genes from study species to test gene function and allelic effects. Uses heterologous expression system to isolate gene effects.

Typical Equipment

• transformation vectors
• plant growth chambers
• HPLC systems

Output Measurements

• transgenic plant glucosinolate levels
• gene expression patterns
• chemical phenotypes

Papers Using This Protocol (1)