Electrospray mass spectrometry alkaloid quantification (Plantae)

Local Knowledge Graph (13 entities)

Knowledge graph centered on Electrospray mass spectrometry alkaloid quantification (Plantae) with 13 nodes and 26 connections. Top connected: plant-pollinator mutualism, pollinator foraging behavior, Norditerpene alkaloid concentrations in tissues an, glucosinolates, Resource Availability Hypothesis.

Description

Plant tissues were extracted in methanol and analyzed by electrospray mass spectrometry to identify and quantify norditerpene alkaloids. Mass spectra were recorded over 150-800 m/z range and alkaloid concentrations calculated based on relative abundance of internal standard.

Typical Equipment

• Electrospray mass spectrometer
• Wiley mill
• Retsch mixer mill MM301
• Centrifuge

Output Measurements

• alkaloid concentrations
• alkaloid identities
• mass spectra

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