D1/D2 rRNA gene sequencing (Ranunculaceae)

Local Knowledge Graph (13 entities)

Knowledge graph centered on D1/D2 rRNA gene sequencing (Ranunculaceae) with 13 nodes and 33 connections. Top connected: plant-pollinator mutualism, pollinator foraging behavior, Artificial nectar robbing, nectar-inhabiting bacteria, standard microbiology protocols.

Description

Yeast isolates were cultured on chloramphenicol-supplemented YM agar and identified through PCR amplification and sequencing of the D1/D2 domains of the large subunit nuclear ribosomal RNA gene using primers NL1 and NL4.

Typical Equipment

• YM agar
• chloramphenicol
• REDExtract-N-Amp Tissue PCR kit
• DNAEngine Thermal Cycler

Output Measurements

• yeast species identity
• DNA sequences

Papers Using This Protocol (1)