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Fish mucus extraction and processing (Baetidae)

Subcategory: chemical extraction
Standard reference: Landeira-Dabarca, Sieiro, & Alvarez, 2013
Papers: 2 | Mentions: 2

Local Knowledge Graph (15 entities)

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Knowledge graph centered on Fish mucus extraction and processing (Baetidae) with 15 nodes and 43 connections. Top connected: antipredator behavior, interspecific competition, alarm communication, nonconsumptive effects, Microcosm predator avoidance bioassay.

Description

Method for collecting fish skin mucus by gentle rubbing in plastic bags followed by freeze-drying and chemical characterization of mucus components including glycosaminoglycans, hexosamine, and uronic acid.

Typical Equipment

  • cotton
  • plastic bags
  • micropipette
  • preweighed vials
  • speed-vacuum freeze dryer

Output Measurements

  • mucus dry weight
  • water content
  • sulfated sugar concentration
  • hexosamine concentration
  • uronic acid concentration

Papers Using This Protocol (2)

article

Mayflies avoid sweets: fish skin mucus amino sugars stimulate predator avoidance behaviour of <i> Baetis </i> larvae

2019Animal Behaviourusing
article

Origin and specificity of predatory fish cues detected by Baetis larvae (<i>Ephemeroptera; Insecta</i>)

2014Animal Behaviourusing