DNA barcoding (Apidae)

Local Knowledge Graph (17 entities)

Knowledge graph centered on DNA barcoding (Apidae) with 17 nodes and 32 connections. Top connected: Genes Suggest Ancestral Colour Polymorphisms Are S, intraspecific variation, ecological speciation, gene coalescence, metabarcoding.

Description

Standard DNA extraction, amplification and sequencing of mitochondrial COI barcode region and nuclear PEPCK gene from museum and field specimens to generate phylogenetic data for species delimitation.

Typical Equipment

• standard DNA extraction protocols
• PCR primers
• sequencing equipment
• MEGA version 6.06
• EMBOSS Transeq

Output Measurements

• COI sequences
• PEPCK sequences
• unique haplotypes

Papers Using This Protocol (1)