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Qiagen DNeasy Blood and Tissue Kit protocol (Limnephilidae)

Subcategory: molecular
Standard reference: Qiagen DNeasy Blood and Tissue kit manufacturer's protocol
Papers: 1 | Mentions: 1

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Knowledge graph centered on Qiagen DNeasy Blood and Tissue Kit protocol (Limnephilidae) with 12 nodes and 22 connections. Top connected: gene coalescence, metabarcoding, molecular evolution rate, PCR primer screening, mosaic genome evolution.

Description

Extraction of total genomic DNA from individual caddisfly larvae using Qiagen DNeasy Blood and Tissue kit with automated QiaCube processing after manual tissue homogenization.

Typical Equipment

  • Qiagen DNeasy Blood and Tissue kit
  • ceramic mortar and pestle
  • Qiagen QiaCube extraction robot
  • NanoDrop 2000 spectrophotometer

Output Measurements

  • total genomic DNA
  • DNA concentration
  • DNA quality metrics

Papers Using This Protocol (1)

article

Identifying PCR primers to facilitate molecular phylogenetics in Caddisflies (Trichoptera)

2015Zoological Systematicsusing