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Bee gut bacterial isolation and genome sequencing (Orbaceae)

Subcategory: microbial isolation
Standard reference: Kwong WK, Moran NA. 2016. Gut microbial communities of social bees. Nat Rev Microbiol 14:374–384
Papers: 1 | Mentions: 1

Local Knowledge Graph (13 entities)

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Knowledge graph centered on Bee gut bacterial isolation and genome sequencing (Orbaceae) with 13 nodes and 23 connections. Top connected: horizontal gene transfer, host-microbiome interactions, Maximum likelihood phylogenetic analysis, phosphotransferase system, metabolic complementarity.

Description

Isolation of pure bacterial cultures from bee gut homogenates followed by whole genome sequencing using Illumina MiSeq and PacBio platforms with subsequent assembly and annotation.

Typical Equipment

  • 10 mM MgSO4
  • heart infusion agar
  • Illumina MiSeq
  • PacBio RS II

Output Measurements

  • bacterial isolate cultures
  • genome sequences
  • phylogenetic relationships

Papers Using This Protocol (1)

article

Metabolism of toxic sugars by the bee gut symbiont <i>Gilliamella apicola</i>

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