Plant tissue genomic DNA extraction for high molecular weight DNA (Brassicaceae)

Local Knowledge Graph (14 entities)

Knowledge graph centered on Plant tissue genomic DNA extraction for high molecular weight DNA (Brassicaceae) with 14 nodes and 33 connections. Top connected: Young inversion with multiple linked QTLs under se, local adaptation, ecological speciation, quantitative genetics, Principal Component Analysis (Brassicaceae).

Description

Extraction of high molecular weight nuclear DNA from etiolated plant seedlings with minimal organellar contamination. Uses nuclei isolation followed by DNA purification for downstream genome sequencing.

Typical Equipment

• MS liquid medium
• MEB buffer
• commercial blender
• TE buffer

Output Measurements

• high molecular weight genomic DNA

Papers Using This Protocol (1)