RNA-seq differential expression analysis (Sciuridae)

Local Knowledge Graph (15 entities)

Knowledge graph centered on RNA-seq differential expression analysis (Sciuridae) with 15 nodes and 31 connections. Top connected: mark-recapture (Sciuridae), dispersal, philopatry, Gene expression shifts in yellow-bellied marmots p, transcriptomics.

Description

Bioinformatics pipeline for processing RNA-seq reads including quality control, mapping to reference genome, and statistical analysis of differential gene expression using mixed models.

Typical Equipment

• Trim Galore
• TopHat2 v2.1.0
• EMMERML
• LIMMA package
• R version 3.3.1

Output Measurements

• differentially expressed genes
• fold changes
• q-values

Papers Using This Protocol (1)