RNA sequencing (Sciuridae)

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Knowledge graph centered on RNA sequencing (Sciuridae) with 22 nodes and 52 connections. Top connected: mark-recapture (Sciuridae), interspecific competition, thermal performance, dispersal, philopatry.

Description

RNA extraction from blood samples, library preparation, and high-throughput sequencing on Illumina platforms followed by read mapping and differential gene expression analysis using mixed models.

Typical Equipment

• TruSeq Library Prep Kit v2
• Illumina HiSeq2000
• Illumina HiSeq4000
• TopHat2
• HT-Seq

Output Measurements

• gene expression levels
• transcript counts
• differential expression statistics

Papers Using This Protocol (2)