Honeydew collection and chemical analysis

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Knowledge graph centered on Honeydew collection and chemical analysis with 21 nodes and 73 connections. Top connected: phenological mismatch, interspecific competition, snow cover duration, mutualistic relationships, Flowering phenology census.

Description

Collection of aphid honeydew on aluminum foil substrates followed by LC-MS/MS analysis to quantify sugar and amino acid composition as indicators of plant quality and ant-aphid mutualism benefits.

Typical Equipment

aluminum foil squares
stereoscope
LC-MS/MS system
ImageJ

Output Measurements

honeydew deposition rate
sugar concentrations
amino acid concentrations

Papers Using This Protocol (2)

article

Early snowmelt reduces aphid abundance <i>Aphis asclepiadis</i> by creating water stressed host plants <i>Ligusticum porteri</i> and altering interactions with ants

2020Arthropod-Plant Interactionsintroducing

article

Elevated temperatures alter an ant aphid mutualism

2019Entomologia Experimentalis et Applicatausing