Synteny-based chromosome scaffolding (Pieridae)

Local Knowledge Graph (18 entities)

Knowledge graph centered on Synteny-based chromosome scaffolding (Pieridae) with 18 nodes and 33 connections. Top connected: The genome of the Margined White butterfly (Pieris, evolutionary trap, CRISPR-Cas9 gene editing (Pieridae), chromosomal inversion, genome polishing.

Description

Using synteny analysis with a closely related reference genome to arrange contigs into chromosome-level scaffolds using MUMmer alignment and RagTag scaffolding.

Typical Equipment

• nucmer (MUMmer4, v.4.0.0beta2)
• RagTag

Output Measurements

• pseudo-chromosomal assembly
• synteny blocks
• chromosome assignments

Papers Using This Protocol (1)