PoolSeq vs individual short-read polishing comparison (Pieridae)

Local Knowledge Graph (18 entities)

Knowledge graph centered on PoolSeq vs individual short-read polishing comparison (Pieridae) with 18 nodes and 33 connections. Top connected: The genome of the Margined White butterfly (Pieris, evolutionary trap, CRISPR-Cas9 gene editing (Pieridae), chromosomal inversion, genome polishing.

Description

Comparative analysis of genome assembly improvement using pooled vs individual Illumina short reads for polishing long-read assemblies, assessed using BUSCO completeness metrics.

Typical Equipment

• Illumina HiSeq platform
• Pilon (v.1.23)
• SeqKit
• NextGenMap

Output Measurements

• BUSCO scores
• assembly completeness
• ortholog hit ratios

Papers Using This Protocol (1)