PCR-based complete viral genome recovery (Sciuridae)

Local Knowledge Graph (13 entities)

Knowledge graph centered on PCR-based complete viral genome recovery (Sciuridae) with 13 nodes and 24 connections. Top connected: metagenome-assembled genome, gene coalescence, Diverse cressdnaviruses and an anellovirus identif, rolling circle amplification, Viral metagenomics from mammalian feces (Sciuridae).

Description

Method using abutting primers designed from contigs to amplify and recover complete circular viral genomes, followed by cloning and Sanger sequencing for verification.

Typical Equipment

• Kapa HiFi HotStart DNA polymerase (Kapa Biosystems, USA)
• pJET 1.2 vector
• Escherichia coli DH5-alpha competent cells

Output Measurements

• complete viral genome sequences

Papers Using This Protocol (1)