BCMA3 Sanger sequencing and structural variant analysis (Brassicaceae)

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Description

Sanger sequencing of BCMA3 alleles from natural accessions combined with Nanopore long-read genome assembly to identify structural genetic variants affecting glucosinolate biosynthesis.

Typical Equipment

• Sanger sequencing platform
• Nanopore sequencing platform

Output Measurements

• BCMA3 sequence variants
• structural variants
• amino acid substitutions

Papers Using This Protocol (2)