microsatellite DNA analysis (Formicidae)

Local Knowledge Graph (9 entities)

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Knowledge graph centered on microsatellite DNA analysis (Formicidae) with 9 nodes and 15 connections. Top connected: kin selection, polygamous mating system, microsatellite DNA analysis, sex allocation theory, relatedness asymmetry.

Description

DNA extraction from ant specimens followed by PCR amplification of microsatellite loci and relatedness estimation using maximum likelihood methods based on population allele frequencies.

Typical Equipment

microcentrifuge tubes
hand drill
lysis buffer
PCR reagents
Techne Laboratories thermal cycler
polyacrylamide gels

Output Measurements

relatedness coefficients
allele frequencies
genetic diversity estimates

Papers Using This Protocol (1)

student_paper

Assessing the Impact that the Keystone Species, the Red Naped Sapsucker (<i> Sphyrapicus nuchalis </i>), has on the Community of Species in the East River

2022using