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soil enzyme activity assay

Subcategory: enzymatic
Standard reference: Saiya-Cork and Sinsabaugh (2002)
Papers: 2 | Mentions: 3

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Knowledge graph centered on soil enzyme activity assay with 28 nodes and 96 connections. Top connected: elevational gradients, carbon cycling, ecosystem function, nitrogen retention, soil respiration.

Description

Measurement of six different soil extracellular enzyme activities using microplate spectrophotometry to understand microbial activity and nutrient cycling processes in response to plant removal treatments.

Typical Equipment

  • Synergy HT microplate spectrophotometer
  • sodium acetate buffer

Output Measurements

  • α-glucosidase activity
  • β-glucosidase activity
  • cellobiohydrolase activity
  • β-Xylosidase activity
  • β-N-acetylglucosamini-dase activity
  • acid phosphatase activity

Papers Using This Protocol (2)

article

Soil signals of key mechanisms driving greater protection of organic carbon under aspen compared to spruce forests in a North American montane ecosystem

2025Biogeosciencesusing
article

Plant removal across an elevational gradient marginally reduces rates, substantially reduces variation in mineralization

2022Ecologyusing