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microsatellite analysis (Animalia)

Subcategory: genomic
Standard reference: Miller et al. 1988; Schlötterer 1998
Papers: 4 | Mentions: 15

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Knowledge graph centered on microsatellite analysis (Animalia) with 40 nodes and 88 connections. Top connected: neighborhood effects, population dynamics, trait variation, social phenotype, courtship displays.

Description

Pooled whole genome sequencing approach using DNA from multiple individuals per population to identify SNPs associated with environmental variables and population differentiation patterns.

Typical Equipment

  • collection containers
  • ethanol
  • CTAB solution
  • Qubit
  • Illumina HiSeq3000
  • FASTQC
  • TRIMMOMATIC
  • BWA-MEM
  • SAMTOOLS
  • GATK

Output Measurements

  • SNP frequencies
  • FST values
  • population genetic statistics

Papers Using This Protocol (4)

article

Evolution and genomic basis of the plant penetrating ovipositor: a key morphological trait in herbivorous Drosophilidae

2022Proceedings of the Royal Society Busing
article

Cold adaptation drives population genomic divergence in the ecological specialist, <i> Drosophila montana </i>

2021Molecular Ecologyusing
article

Phylogeographic patterns in <i>Drosophila montana</i>

2007Molecular Ecologyusing
article

Microsatellite-based species identification method for <i>Drosophila virilis</i> group species

2007Hereditasintroducing