16S rRNA gene sequencing (Nymphalidae)

Local Knowledge Graph (12 entities)

Knowledge graph centered on 16S rRNA gene sequencing (Nymphalidae) with 12 nodes and 24 connections. Top connected: plant-pollinator mutualism, pollinator foraging behavior, nectar-inhabiting bacteria, The dispersal of microbes among and within flowers, Butterfly-mediated microbial dispersal experiment (Nymphalidae).

Description

Standard protocol to isolate, culture, and identify bacteria using genomic DNA extraction, 16S rRNA gene sequencing, and NCBI database comparison.

Typical Equipment

• HiMedia nutrient agar
• DNeasy Blood and Tissue Kit
• Hi-Seq platform

Output Measurements

• bacterial species identification

Papers Using This Protocol (1)