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YM agar preparation (Nymphalidae)

Subcategory: microbial enumeration
Standard reference: Suh, Zhang, Nguyen, Gross, & Blackwell, 2008
Papers: 2 | Mentions: 3

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Knowledge graph centered on YM agar preparation (Nymphalidae) with 15 nodes and 36 connections. Top connected: plant-pollinator mutualism, pollinator foraging behavior, species abundance, nectar-inhabiting bacteria, The dispersal of microbes among and within flowers.

Description

Method to quantify microbial load on different butterfly body parts by direct plating on agar media. Samples tarsi/proboscis by dragging across plate and thorax by washing technique.

Typical Equipment

  • YM agar plates
  • sterile needle-tip forceps
  • autoclave envelopes

Output Measurements

  • microbial colony counts by body part