LC-MS plant metabolomics (Poaceae)

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Knowledge graph centered on LC-MS plant metabolomics (Poaceae) with 25 nodes and 81 connections. Top connected: climate change effects on species interactions, thermal performance, climate change experiments, biogeochemical reactions, plant-microbial coupling.

Description

Extraction of plant metabolites using methanol/acetonitrile/water solvent followed by liquid chromatography-mass spectrometry analysis. Characterizes plant metabolomic profiles for correlation with fungal communities.

Typical Equipment

• HPLC grade solvents
• Synergi 2.5 μm reverse-phase Hydro-RP column
• Exactive™ Plus Orbitrap Mass Spectrometer

Output Measurements

• metabolite abundance profiles

Papers Using This Protocol (1)